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2009.04.27
HIV-1 p24 ELISA Kit - Now available!!

80-001

HIV-1 p24 ELISA Kit – Now available!!

HIV-1 p24 ELISA Kit  “BioAcademia” 

Determination of the amount of HIV-1 Gag p24 antigen

- In vitro Research Use Only -

This kit can measure the amount of HIV-1 Gag p24 antigen in cell culture medium handily by a sandwivh ELISA (Enzyme Linked Immunosorbent Assay) method.  P24 antigen is a structure protein of HIV-1, so when this is measured, it’s possible to presume the virus amount in the sample.

 

This kit is made based on the principle of a sandwich ELISA method using an antibody coated plate as a solid phase, a biotinyl antibody and a peroxidase-labeled streptavidin.

HIVp24-1.png


Advantage of this kit

p24 of subtype AE can be measured at the same sensitivity as subtype B, because affinity purified polyclonal antibody raised against the full length recombinat p24 is used.


Risk-free kit, because neither patient sera nor active virus products ae used.


Assay can be performed at room temparature.

HIVp24-2.png


Figure  Standard curve of HIV-1 p24 measurement by the 2 hour assay


Technical Data

 Minimum detection limit

0.9pg/mL

Typical standard curve

 

HIV(1).jpg

 


Specifications

Product name

HIV-1 p24 ELISA Kit “BioAcademia”

Product code

80-001

Format

Sandwich EIA

Package

96 wells

Sample type

Cell cuture medium

Sample size

200 uL

Range

0 – 100 pg/mL

Storage

4

 

HIV related products

 

Code# Product Name Package Strage Species Protocol MSDS

Immunoassays

EIA and ELISA
80-001 HIV-1 p24 ELISA Kit “BioAcademia” 96wells 4℃ HIV-1, Human immunodeficiency virus type 1 Protocol

Reagents / Proteins

HIV-1 Recombinant Proteins
05-007 HIV-1 Gag p15 20ug -20℃ Protocol
05-008 HIV-1 Gag p15 100ug -20℃ Protocol
05-003 HIV-1 Gag p17 20ug -20℃ Protocol
05-004 HIV-1 Gag p17 100ug -20℃ Protocol
05-005 HIV-1 Gag p24 20ug -70℃ Protocol
05-006 HIV-1 Gag p24 100ug -70℃ Protocol
05-009 HIV-1 Gag p55 20ug -70℃ Protocol
05-010 HIV-1 Gag p55 100ug -70℃ Protocol
05-011 HIV-1 Nef 20ug -20℃ Protocol
05-012 HIV-1 Nef 100ug -20℃ Protocol
05-001 HIV-1 Reverse transcriptase 200units -20℃ Protocol
05-002 HIV-1 Reverse transcriptase 1000units -20℃ Protocol

Antibodies

Viruses and Infectious Agents
65-011 anti-HIV-1 Gag p15 antibody, rabbit serum 50uL 4℃ rabbit Protocol
65-012 anti-HIV-1 Gag p15 antibody, rabbit serum 250uL 4℃ rabbit Protocol
65-010 anti-HIV-1 Gag p17 antibody, guinea pig serum 50uL 4℃ guinea porcine Protocol
65-008 anti-HIV-1 Gag p17 antibody, rabbit serum 50uL 4℃ rabbit Protocol
65-009 anti-HIV-1 Gag p17 antibody, rabbit serum 250uL 4℃ rabbit Protocol
65-006 anti-HIV-1 Gag p24 antibody, guinea pig serum 50uL 4℃ guinea porcine Protocol
65-004 anti-HIV-1 Gag p24 antibody, rabbit serum 50uL 4℃ rabbit Protocol
65-005 anti-HIV-1 Gag p24 antibody, rabbit serum 250uL 4℃ rabbit Protocol
65-013 anti-HIV-1 Gag p55 antibody, rabbit serum 50uL 4℃ rabbit Protocol
65-014 anti-HIV-1 Gag p55 antibody, rabbit serum 250uL 4℃ rabbit Protocol
65-017 anti-HIV-1 Nef antibody, guinea pig serum 50uL 4℃ guinea porcine Protocol
65-015 anti-HIV-1 Nef antibody, rabbit serum 50uL 4℃ rabbit Protocol
65-016 anti-HIV-1 Nef antibody, rabbit serum 250uL 4℃ rabbit Protocol
65-003 anti-HIV-1 Reverse Transcriptase antibody, guinea pig serum 50uL 4℃ guinea porcine Protocol
65-001 anti-HIV-1 Reverse Transcriptase antibody, rabbit serum 50uL 4℃ rabbit Protocol
65-002 anti-HIV-1 Reverse Transcriptase antibody, rabbit serum 250uL 4℃ rabbit Protocol


 

1008_80-001_p.pdf

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