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DSP Rapid Kit (Diarrheic Shellfish Poison Test)
New Generation Assay for all Diarrheic Shellfish Poisons

 

 

 

 

 

 

 

BT0101 DSP Rapid kit (96wells)
BT0102 DSP Rapid kit (48wells)

1.    What is DSP?

Diarrheic Shell­fish Poison (DSP) is caused by the ingestion of shell­fish contaminated by toxic dino‑agellates. DSP has been observed worldwide and caused problems to public health and the shellfi­sh industry.
So periodical monitoring of DSP level in shellfish is necessary in each country and area.

 

2.    Characteristics - New Generation Assay

The assay is based on the inhibition of the protein phosphatase (PP2A) by DSP toxins (okadaic acid and dinophysistoxin :OA and DTXs), which is the next generation assay  principal to ELISA and mass spectrometry.
So compare to Immunoassay such as ELISA, DSP Rapid Kit superior to following points;

-    High sensitivity
The assay is based on the inhibition of the protein phosphatase (PP2A) by DSP toxins (OA and DTXs). This principal is same as in-vivo toxicity process of DSP, so this assay measure not only DSP but also entire toxicity, that is ideal evaluation methods for DSP.
Minimum detection limit is 0.036ug/g, and minimum quantification limit is 0.056ug/g (data not shown).

-    High specificity
Enzyme inhibition assay eliminate cross reaction and non-specific reaction, that EIA and ELISA have

-    Rapid and Easy
DSP Rapid Kit adapts homogeneous one-step assay, without complicated wash and multiple incubation steps.
This assay takes only 30 minutes as incubation time.

3.    Assay Principal

PP2A can hydrolyze a colorless artificial substrate, p-nitrophenyl phosphate (p-NPP), and produces the yellow color of p-nitrophenol (p-NP) in the alkaline solution. The intensity of the color is proportional to the enzyme activity and the absorbance is measured at 405 nm. The concentration of DSP toxins in the sample is calculated from the standard curve produced using known concentrations of OA.

 

4.    Procedure

Data calculation sheet in Excel format is available. Click here



Instruction video is available. Click here.

5.    Specifications

Product descriptions and package inserts are available in following.

BT0101 DSP Rapid kit (96wells)
BT0102 DSP Rapid kit (48wells)

 

6.    Application

-    Food safety test
-    Environmental pollution evaluation
This assay measure not only DSP but also entire toxicity of DSP (OA and DTXs).