News
- 2009.10.22
- YK240 Corticosterone EIA - circadian rhythm and stress biomarker
For research use only
1. What is Corticosterone?
Corticosterone (C21H30O4, also called 11beta, 21-Dihydroxyprogesterone, Reichstein’s Substance H, or Kendall’s Compound B) is, like cortisol and cortisone, a glucocorticoid hormone secreted from the cortex of adrenal gland.
It is a primary glucocorticoid in mice and rats and other animals (such as rabbits, birds, amphibians, and reptilians) in which the 17alfa-hydroxylase is supposed not to exist in adrenal gland.
Corticosterone is produced under the control of ACTH and the production has a circadian rhythm with peak levels in the latter portion of the day in nocturnal animals like rats and is believed to play a decisive role in sleep-wake cycle. Corticosterone can be used as a non-invasive biomarker of stress study through the collection of urine and feces to avoid corticosterone increase of blood levels which is caused by normal invasive methods.
2. Specification
YK240 Corticosterone EIA kit is a quite useful tool for further development of corticosterone research.
This assay kit is newly developed by our laboratory provides a tool for direct determination of corticosterone in blood by simple dilution of blood samples with the diluent included in the kit. Any complicated extraction processes in sample preparation like other commercial kit are not needed. Furthermore, assays using the kit can be completed within a short period.
| YK240 Corticosterone EIA Kit | |
Contents | |
| ▼ | The assay kit range: 0.21-50ng/mL. | 1) | Antibody Coated Plate |
| ▼ | The assay running time: 2h. + 0.5 h. | 2) | Corticosterone Standard |
| ▼ | Maximum measurable samples: 41 in duplicate | 3) | HRP-Labeled Corticosterone |
| ▼ | Test sample: plasma, serum, urine, and cell or tissue culture supernatant etc. | 4) | Specific Antibody |
| 5) | TMB Substrate | ||
| ▼ | The 96-well plate in the kit is consisted of 8-wells strips, so that the kit can be used dividedly in strips. | 6) | Reaction Stopping Solution |
| |
Intra-assay %CV: 2.5~4.7 | 7) | Buffer Solution |
| ▼ | Inter-assay %CV: 7.5~9.8. | 8) | Sample Diluent |
| ▼ | 9) | Wash Solution Concentrated | |
| |
Store all the components in the kit at 2-8℃. The kit is stable under the condition for 6 months from the date of the manufacturing. | 10) | Adhesive Foil |
| ▼ | The expiry date is stated on the package. | |
|
| |
Cross reactivities of the antibody used in the kit.
|
Compound |
Cross Reactivity （％） |
|
Corticosterone |
100 |
|
11-Deoxycorticosterone |
<15.5 |
|
Progesterone |
<5.9 |
|
Androstenedione |
<5.4 |
|
Testosterone |
<3.9 |
|
Aldosterone |
<1.7 |
|
Cortisol |
<0.5 |
|
Cortisone |
<0.4 |
|
DHEA |
<0.05 |
|
Estradiol |
0 |
|
Cholesterol |
0 |
3. Assay principal
This EIA kit for determination of corticosterone is based on a competitive enzyme immunoassay using combination of specific antibody to corticosterone and corticosterone- horseradish peroxidase (HRP) conjugate (HRP-labeled corticosterone) system.
4. Kit contents
| Component | Form | Quantity | Main Ingredient | |
| 1 | Antibody Coated Plate | microtiter plate | 1 plate (96 wells) | Goat anti rabbit IgG |
| 2 | Corticosterone Standard | lyophilized powder | 1 vial (50ng) | Synthetic corticosterone |
| 3 | HRP-Labeled Corticosterone | lyophilized powder | 1 vial | HRP conjugated corticosterone |
| 4 | Specific Antibody | liquid | 1 bottle (7 mL) | Rabbit anti corticosterone antibody |
| 5 | TMB Substrate | liquid | 1 bottle (12 mL) | 3,3’,5,5’-Tetramethylbenzidine (TMB) |
| 6 | Reaction Stopping Solution | liquid | 1 bottle (12 mL) | 1M Sulfuric acid |
| 7 | Buffer Solution | liquid | 1 bottle (10 mL) | BSA-containing PBS buffer |
| 8 | Sample Diluent | liquid | 1 bottle (50 mL) | A specially formulated displacer of CBG |
| 9 | Wash Solution Concentrated | liquid | 1 bottle (25 mL) | Concentrated saline |
| 10 | Adhesive Foil | 2 sheets |
5. Technical data
Assay range is 0.21-50 ng/mL.
For detail, see package insert. Clock here to package insert.
6. Research field
- Circadian rhythm for mice and rats and other animals (such as rabbits, birds, amphibians, and reptilians).
- As urinary stress marker.
- Impairment of long-term memory retrieval.
- Dietary restrictions.
- Burn injuries, etc.
7. Product description
Click here to jump to product description
