Products | SCETI BIOSCIENCE Export | SCETI BIOSCIENCE Export

1st Incubation 1 hr, 2nd incubation over night, 3rd incubation 30 min

Detection Target name
Bradykinin

Target Synonyms
Bradykinin, Kallidin I

Description
Kallidinogenase as a cardiovascular active substance is one of kininogenases which release kinins by specifically acting on kininogens. It has been widely used in the medical treatment as a cardiovascular drug such as an antihypertensive drug. It is generally acknowledged that the measurement of kinin-degrading enzymes (kininases) and kinin-releasing activity in the bulk substance or preparations is important in the process and quality control in the manufacture of kallidinogenase preparations. Kininases are determined by the bioassay method with bradykinin (BK) as a substrate using the rat uterus or guinea pig ileum. On the other hand, for the measurement of kinin-releasing activity, the bioassay method is used in which the formed kinin is determined using kininogen as a substrate. However, these bioassay methods are complicated in procedure and require a great deal of labor. Therefore, we developed the kit for the assay of BK, MARKIT-A Bradykinin, based on enzyme immunoassay (EIA). However, as this method uses test tubes and requires two centrifugation steps, an improvement of the assay method has been awaited. After intensive studies, we have now developed MARKIT-M Bradykinin, a new kit for the assay of BK, which uses microstrip wells.

Specificity

Bradykinin (BK) : 100%
Lys-BK : 100%
Met-Lys-BK : 100%
T-Kinin : 100%
Tyr-BK : 100%
(Hyp3)-BK : 79%
Des-Arg1-BK : 45%
Des-Arg9-(Leu8)-BK : <0.1%
[1-8]-BK : 0.2%

[1-7]-BK : <0.1%

[1-6]-BK : <0.1%

[1-5]-BK : <0.1%

BK potentiator B : <0.1%

BK potentiator C : <0.1%

AngiotensinⅠ : <0.1%

AngiotensinⅡ : <0.1%

LMW-Kininogen : <0.1%

Kininogen(Bovine Plasma) : <0.1%

Standard curve

 

This product is useful for Hypertension study.

Related Products

Peptides

4002 Bradykinin

4008 Lysyl-Bradykinin

4009-v Bradykinin-Protentiator B (Mamushi)

4010-v Bradykinin-Protentiator C (Mamushi)

4012 Methionyl-Lysyl-Bradikinin

4056-v Tyrosyl-Bradykinin

4065 Des-Arg9-[Leu8]-Bradykinin

4067 Des-Arg9-Bradykinin

4075-v [Tyr8]-Bradykinin

4097 Des-Pro2-Bradykinin

4130 Isoleucyl-Seryl-Bradikinin [T-Kinin]

4175-v [Thi5, 8, D-Phe7]-Bradykinin

4191-v Lysyl-[Hyp3]-Bradykinin

4193-v [Hyp3]-Bradykinin

Reference

(1) Uchida Y. et al.: Jpn. J. Clin. Med., 40 (special fall issue), p772, 1982.

(2) Uchida Y. et al.: Acta Haematol. Jpn., 45, p498, 1982.

(3) Tanimoto T. et al.: Iyakuhin Kenkyu, 16, p839, 1985.

(4) Majima M. et al.: "Development of high sensitive enzyme-linked immunosorbent assay (ELISA) of bradykinin and its application for determination of free kinin levels in human biological fluids." Kinin 93 Brazil, Abstract book, p62, 1993.

(5) Majima M et al.: "Diruetic and natriuretic effect of ebelactone B in anesthetized rats by inhibition of a urinaty carboxypeptidase Y-like kininase." Jpn. J. Pharmacol., 65, p79, 1994.

(6) Sakata Y. et al.: "Bradykinin generation triggered by Pseudomonas proteases facilitates invasion of the systemic circulation by Pseudomonas aeruginosa." Microbiology&Immunology, 40, p415, 1996.

(1)-(3): Available only in Japanese.